Antibody Gives Consistent Staining

September 01, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Anti-mouse MHCII I-Ab eF450

Catalog Number:

48-5320-82

Clone Number:

AF6-120.1

It works great and the staining is strong.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:200, 30 min, 4C

Blocking Agent

1% BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We use this antibody regularly to stain antigen presenting cells from the thymus and the lymph nodes. It works great and the staining is strong.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong staining

The Bad

none

The Bottom Line

Antibody has great signal, highly recommended..

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