Highly Recommended Alexa Fluor 647 Anti-Mouse

August 31, 2021

Microbiology and Immunology
Virginia Commonwealth University
MD/PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-mouse IFN-γ Antibody

Catalog Number:

505814

Clone Number:

XMG1.2

Antibody was used to identify IFNg producing CD8 and CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

perigonadal white adipose tissue, gated on CD8 T cells

Primary Incubation

1:200, overnight at 4C.

Blocking Agent

2.4g2

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry, LSR Fortessa

Results Summary

Stains IFNg producing T cells well with a fairly good spread.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, good titration

The Bad

None

The Bottom Line

Highly recommend.

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