Reliable PE Anti-mouse/human CD11b

August 24, 2021

BlueskyReddit
CCTR
Virginia Commonwealth University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse/human CD11b Antibody

Catalog Number:

101208

Clone Number:

M1/70

Easy to use antibody. Good stain index for particular antigen and fluorophore. Cost effective.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Perigonadal white adipose tissue

Primary Incubation

1:400 dilution, incubated 30 minutes at 4C.

Blocking Agent

2.4g2

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry, LSR Fortessa

Results Summary

Easy to identify CD11b positive cells from negative cells. Using with Siglec-F for eosinophils clearly identifies the population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, good titration, great separation

The Bad

None

The Bottom Line

Highly recommended!

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