Great Antibody for Flow Staining

August 20, 2021

BlueskyReddit
Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Anti-mouse CD11c PE/Cy7

Catalog Number:

25-0114-82

Clone Number:

N418

We use this antibody regularly to stain dendritic cells from the thymus and the lymph nodes. It works great and the staining is strong.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus

Primary Incubation

1:200, 30 min, 4C

Blocking Agent

1% BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We use this antibody regularly to stain dendritic cells from the thymus and the lymph nodes. It works great and the staining is strong.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong staining

The Bad

None

The Bottom Line

Antibody has great signal, highly recommended.

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