Reliable CD163 Antibody for IFA

Molecular Microbiology
Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

CD163 Polyclonal Antibody

Catalog Number:

16646-1-AP

Clone Number:

N/A

CD163 is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily. I am detecting the CD163 presentation in human macrophages before and after treatment.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human macrophage monolayer

Primary Incubation

1:500, RT

Blocking Agent

2% BSA

Secondary Incubation

1:2000 anti-human

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

This antibody helped me finding the altered expression of CD163 in different treatment conditions. IFA of CD163 with this antibody worked quite well. I tried western blot with this antibody first, but did not get any good result, even though the product itself should be working with western blot.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works with IFA

The Bad

DID not work with western blot

The Bottom Line

It works great with IFA, recommend.

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