Biolegend
Brilliant Violet 510™ anti-mouse/human CD44 Antibody
103043
IM7
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at titrating anti-CD44 BV510.
Flow Cytometry
Mouse WT spleen
Surface staining with CD44 BV510 (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
CD44 is expressed by all lymphocytes. We could titrate the antibody, and decided to use it at 1/200.
Reliable and good signal
Nothing bad about it
Great, would buy again