Good Blocking Solution for Western Blotting

Centre for Cellular & Molecular Biology
Project JRF
Postdoctoral Researcher

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Company:

Li-Cor

Product Name:

Intercept blocking buffer

Catalog Number:

927-70001

This Li-Cor TBS blocking buffer is a ready made buffer of immunoblotting assays. This buffer is very efficient in blocking non specific signals when using chemiluminescent or near infrared detection techniques. The efficiency of this buffer is significantly higher that lab made BSA or skimmed milk blocking solution.

Experimental Design and Results Summary

Application

Western blot blocking solution.

Starting Material

Protein immobilized PVDF or Nitrocellulose membrane

Tips

The blocking buffer after every use can be used for making primary or secondary antibody solution.

Results Summary

Sharp specific protein bands with minimum or less background signals

DOI or PMID #

N/A

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Summary

The Good

Reduced background signals, ready to use and cost effective

The Bad

Nothing significant.

The Bottom Line

One of the best immunoblot blocking solution in the market.

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