Anti-mouse SLAM6 (Ly-108 or CD352) Gives OK Results

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biosciences

Product Name:

BV711 Mouse Anti-Mouse Ly-108

Catalog Number:

740823

Clone Number:

13G3

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at titrating anti-mouse SLAM6 to use in future experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

B6 mouse spleen

Primary Incubation

surface staining with anti-SLAM6 BV711, 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain lymphocytes with anti-ly108 at several dilutions, but the best dilution is 1/100.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable

The Bad

The signal is weak.

The Bottom Line

It can be used.

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