BosterBIO
Anti-cd11b ITGAM rabbit monoclonal
M00144-1
Monoclonal GC-9
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My goal is to isolate primary cells and confirm the purity by looking at the expression of cd11b in primary isolated microglias.
Immunofluorescence
Primary human microglia
1:200
Methanol
Anti rabbbit
None
Microscopy.
The immunofluorescence staining of cd11 in human microglia gave week signal, although based on the morphology the isolated cell cultures seemed to be pure microglias.
Easy to optimize
Weak signal.
Can be used at higher concentration 1:10-1:100 or alternating the fixation protocol.