Reliable a-CD137 Antibody

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD137 Monoclonal Antibody (4B4-1), Alexa Fluor 647

Catalog Number:

A51019

Clone Number:

4B4-1

CD8+ T-cells were co-cultured with different cell lines to estimate the specificity and reactivity of the T-cells. CD137 was used as a biomarker for T-cell activation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD8+ T-cells co-cultured with the K562 cell line

Primary Incubation

1:100, 20 min, RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

The staining of the antibody gave a decent signal and a clear separation of the populations was observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

Larger quantities not available

The Bottom Line

Works well and it's quite cheap, we'll keep using this one.

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