Reliable Western Ladder

University of Alabama
Urology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio Rad pharmaceuticals

Product Name:

PrecisionPlus Protein WesternC Blotting Standards

Catalog Number:

1610376

For every western blot in our lab, we use this ladder. It is pre-stained, strep tagged and has 3 pink reference bands at 25, 50 and 75 kD. it helps in identifying if electrophoresis has produced separation and transferring efficiency. Loading 7ul is enough to produce a strong ladder.

Experimental Design and Results Summary

Application

Western blotting

Starting Material

N/A

Tips

Load 5-7ul, leave a well clear next to the ladder for a clean blot

Results Summary

Use as a reference to keep track of electrophoretic separation

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easily visible when the gel runs.

The Bad

Expensive compared to unstained versions.

The Bottom Line

No conjugate is needed to detect the ladder and helps identify proteins based on weight from 10 to 250 kD

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