Good Microglial Marker

Urology
University of Alabama
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-TMEM119 antibody

Catalog Number:

ab209064

Clone Number:

28-3

Spinal cord frozen sections have minimal amount of microglia. we need a very specific marker for this purpose. this antibody consistently works every time. it is suitable for both frozen and paraffin sections. host is rabbit and reacts with mouse.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

mouse

Primary Incubation

1:300 for overnight at 4C

Blocking Agent

5% BSA in PBS with 1% donkey serum

Secondary Incubation

1:1000 for 1 hour room temperature

Tertiary Incubation

None

Detection

Immunofluoresence

Results Summary

Excellent antibody with very high specificity

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good microglial distinction.

The Bad

Nothing yet.

The Bottom Line

Highly applicable in situations where microglial number is low. Will buy again.

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