Good Anti-Mouse CD8

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Miltenyi Biotec

Product Name:

CD8a Antibody, anti-mouse, REAfinity

Catalog Number:

130-122-017

Clone Number:

REA601

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at titrating anti-CD8.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse WT spleen

Primary Incubation

surface staining with CD8 Viogreen (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

The figure shows the result of the titration. For the majority of the applications 1/400 is probably enough.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High quality and accessible

The Bad

NA

The Bottom Line

pretty good option to have

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