Biolegend
PE/Cyanine7 anti-mouse CD274 (B7-H1, PD-L1) Antibody
124313
10F.9G2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining PDL1 in plasma cells.
Flow Cytometry
Mouse WT spleen
Surface staining with PD-L1 PE-Cy7 (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometry
Plasma cells (CD138hi) express higher amounts of PDL1 than B cells
10.1016/j.immuni.2018.06.007
It works.
It could be brighter.
Gives good results and the price point is reasonable.