Biolegend
APC anti-mouse CD272 (BTLA) Antibody
134809
8F4
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. Here, we aimed at staining BTLA in B and T cells.
Flow Cytometry
mouse spleen
surface staining with anti-BTLA APC (1/150), 20 min at 4C
Fc block (2.4G2 clone)
NA
Flow cytometer
As it can be observed in the figure, we can stain BTLA in B cells, and this molecule was not expressed by CD4 T cells.
Acessible
It could be brighter
I recomend it