Biolegend
PE anti-human IL13 Antibody
501903
JES10-5A2
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This antibody was used to identify the expression of IL13 on Jurkat cells. This particular clone of Ab was found to bind best to our mutated version IL13 and provide us with bright, clean FACS staining of live cells
Flow Cytometry
Jurkat Cells and PBMCs
5ul (1/10) of IL13 (JES10-5A2) PE antibody incubated with cells at 4 deg for 30 minutes.
N/A
LSR II
This antibody worked amazingly well in FACS analysis of cells overexpressing IL13. Minimal background staining. This product enabled us to analyse expression of our marker antigen and was used in subsequent cytotoxicity assays.
Bright and high quality signal
Amazing antibody. Has so far worked well with different cell lines as well as primary cells.