Proteintech
Beta Actin Polyclonal antibody
20536-1-AP
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We ordered this actin antibody from Proteintech and used it as a loading control in our western blot assays when we detected target proteins within mammalian cells. The antibody works great and it usually gives strong signal at 1:2000 dilution in our assay.
Western Blot
human cell lines
1:2000 for 2 h at room temperature
5% skim milk in PBS
1:5000 dilution of secondary antibody for 1 hour at room tempersture
N/A
HRP chemiluminescence
The antibody always gives a nice and specific actin band in our assays.
Very sensitive and specific
Nothing.
We highly recommend this product.