Anti-Hu/Mo CD49f Gives Great Results

June 23, 2021

BlueskyReddit
Research Scientist
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Anti-Human/Mouse CD49f PerCP-eFluor 710

Catalog Number:

46-0495-82

Clone Number:

N/A

Cervical cancer stem cells were evaluated for marker expression using CD49f by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human cervical cancer cells

Primary Incubation

0.2 mg/mL, 1 hour at 4 degrees

Blocking Agent

ALDEFLUOR Assay Buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Flow cytometry revealed positive expression of CD49f in cervical cancer cells from patient tissue, indicating potential cancer stem cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very easy to use.

The Bad

Optmization may be needed depending on sample.

The Bottom Line

This is a fairly reliable antibody for expression in cervical cancer specimens.

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