ABCAM
Anti-cleaved N-terminal GSDMD antibody
ab215203
EPR20829-408
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We investigated the NLRP3 and pyroptosis induction induced bybacterial toxins, and we used this antibody to detect the cleavage of GSDMD in human macrophages.
Western Blot
culture supernatants of THP-1 cells
1:1000; overnight at 4 degree
5% skim milk in PBS
1:5000 for 1 hour at room tempersture
N/A
hrp chemiluminescence
We used this antibody to detect the cleaved GSDMD in culture supernatants of THP-1 cells treated by S. aureus toxins. This antibody works great and can easily detect the cleaved GSDMS in culture supernatants of treated THP-1 cells.
The product is very sensitive and specific.
The product is very expensive.
Expensive but great, and we will buy it again.