Good for Measuring LCK Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Anti-Lck Antibody

Catalog Number:

sc-433

Clone Number:

N/A

Lck is a 56 kDa protein that is found inside specialized cells of the immune system called lymphocytes. Lck is a tyrosine kinase, which phosphorylates tyrosine residues of certain proteins involved in the intracellular signaling pathways of these lymphocytes. Lck expression may contribute to the pathogenesis of some types of neoplastic disease. Here we checked the time course effect of LCK expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is no change in Lck expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This is a antibody and works great for western blot

The Bad

None

The Bottom Line

Works great for western blot. Highly recommended.

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