Good for Detecting Rak

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Rak Antibody

Catalog Number:

sc-166478

Clone Number:

N/A

Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src is the first proto-oncogenic nonreceptor tyrosine kinase characterized in human. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Here we checked the time course effect of Rak expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found no change in Rak expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This is a antibody and works great for western blot

The Bad

None

The Bottom Line

This works good for western blot and signal is great. Highly recommended.

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