Measuring TMPRSS2 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz

Product Name:

Anti-TMPRSS2 Antibody

Catalog Number:

sc-515727

Clone Number:

N/A

TMPRSS2 encodes a protein that belongs to the serine protease family. Serine proteases are known to be involved in many physiological and pathological processes. TMPRSS2 is expressed as a full length form and a cleaved protease domain and its expression is increased by androgenic hormones. TMPRSS2 is also expressed in prostate carcinoma, suggesting that it may play a role in prostate carcinogenesis. Here we checked the time course effect of TMPRSS2 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:500 in 1x TBST prepared in skim milk

Blocking Agent

5% TBST prepared in skim milk

Secondary Incubation

1:4000 in 1x TBST prepared in skim milk

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed time dependent increase of TMPRSS2 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good for western blot.

The Bad

None

The Bottom Line

This works great for western blot and signal is great. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion