Great Anti-Fc-PE Antibody for Flow

August 09, 2021

BlueskyReddit
Surgery
University of Pittsburgh
Research Scientist

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Company:

Millipore Sigma

Product Name:

Anti-Human IgG (γ-chain specific)−R-Phycoerythrin antibody produced in goat

Catalog Number:

P9170

Clone Number:

N/A

To confirm the PSCA binders in CHOK1, flow cytomery was used to detect the binding of PSCA and its binder SF2, SF2-Fc cells compared to the parent cell control (blank) and secondary antibody control. The results showed that SF2-Fc was a good PSCA binder.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CHOK1

Primary Incubation

1/100 on ice for 30'

Blocking Agent

0.1% BSA/PBS

Secondary Incubation

Fc-PE antibody (P9170-.5ML) 1/200 on ice for 30'

Tertiary Incubation

On ice for 30'

Detection

Flow cytometry

Results Summary

To confirm the PSCA binders in CHOK1, flow cytomery was used to detect the binding of PSCA and its binder SF2, SF2-Fc cells compared to the parent cell control (blank) and secondary antibody control. The results showed that SF2-Fc was a good PSCA binder.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody works well.

The Bad

Nothing.

The Bottom Line

Reliable results!

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