Calbindin Monoclonal Antibody Has Great Permeability and Sensitivity

June 04, 2021

BlueskyReddit
Physical Medicine and Rehabilitation
National Taiwan University Hospital and National Taiwan University College of Medicine
Postdoctoral Researcher

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Company:

Genetex

Product Name:

Calbindin (Monoclonal)

Catalog Number:

GTX634833

Clone Number:

GT386

This antibody was used to detect Calbindin expression in mice cerebellum tissue (~250um thickness) by Immunofluorescence (IF).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mice cerebellum (250um slice)

Primary Incubation

1:100, 4℃, 2 day in SignalStain® Antibody Diluent .

Blocking Agent

10% normal goat serum, 1% triton-X100,2.5%DMSO,0.2% sodium azide in PBS

Secondary Incubation

1:200, Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, 4℃, 2 day in SignalStain® Antibody Diluent .

Tertiary Incubation

N/A

Detection

Alexa Fluor 488, zeiss LSM 880

Results Summary

Clear signaling in 250um thickness tissue.

DOI or PMID #

N/A

Additional Notes

After stain, samples( 250um slices) incubated in RapiClear®1.47 60min for tissue clearing, and then mounted (also in RapiClear®) for imaging.

Image Gallery

Summary

The Good

High senesitivity, good permeability for 250~500um thick tissue

The Bad

None

The Bottom Line

We will continue to use this antibody.

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