CellTrace for Cells in Mixed Populations

University of Pittsburgh
Immunology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

Catalog Number:

C34555

Info: Supplier Page 

We used this dye to track a specific cell after mixing them into a coculture for a short experiment. The dye worked as expected and was helpful in identifying the population of cells we wanted to study.

Experimental Design and Results Summary

Application

Cell tracking

Starting Material

D10 T Cells

Tips

Used at 1 ul per 7 million cells and was more than bright enough to detect.

Results Summary

Cells were stained as expected and we were able to differentiate between the stained cells and other cells in the mixed population that we had not stained. The experiment was not long enough to track divisions of cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use, very bright staining at low concentrations

The Bad

None

The Bottom Line

This was easy to use and I'll continue to use this product in my coculture experiments.

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