Thermo Fisher
TRPV4 Polyclonal Antibody
PA5-41066
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This antibody was used to detect TRPV4 expression in mice brain tissue (~250um thickness) by Immunofluorescence (IF).
Immunofluorescence
mice brain (250um slice), Dentate gyrus
1:100, 4℃, 2 day in SignalStain® Antibody Diluent .
10% normal goat serum, 1% triton-X100,2.5%DMSO,0.2% sodium azide in PBS
1:200, Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, 4℃, 2 day in SignalStain® Antibody Diluent .
N/A
Alexa Fluor 488, Zeiss LSM 880
Nice signal in 250um thickness tissue.
High sensitivity, good permeability for 250~500um thick tissue.
Nothing.
We will continue to use this antibody.