Internal Control for EMSA

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Normal mouse IgG

Catalog Number:

sc-2025

Clone Number:

N/A

Immunoglobulins play a key role in the body's immune system. Control immunoglobulin and immunoglobulin conjugates are useful negative controls. Normal sera is offered to be used as blocking reagents. There are four subclasses of IgG: IgG1, IgG2, IgG3, and IgG4. Here we used this mouse IgG antibody for EMSA.

Experimental Design and Results Summary

Applications

Electrophoretic mobility shift assay (EMSA)

Sample

Mouse Nuclear Extract

Primary Incubation

1:200

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

None

Tertiary Incubation

None

Detection

ECL

Results Summary

We found no change after incubated with mouse lysate+IgG Antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and highly specific

The Bad

None

The Bottom Line

This antibody works really great for EMSA and supershift EMSA experiments.

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