Great staining for RORgt

April 25, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

ROR gamma (t) Monoclonal Antibody (AFKJS-9), PE, eBioscience

Catalog Number:

12-6988-82

Clone Number:

AFKJS-9

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse lymph nodes

Primary Incubation

RT 45min 1:250

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Able to detect RORgt+ cells in both lymphocytes and non-lymphocytes

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Consistent staining

The Bad

None

The Bottom Line

Very good clone and fluorochrome

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