Great BCL6 staining

April 25, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE-CF594 Mouse Anti-Bcl-6

Catalog Number:

562401

Clone Number:

K112-91

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

RT 45min 1:250

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Able to detect BCL6+ GC B cells

DOI or PMID #

Additional Notes

Related Categories

Image Gallery

Summary

The Good

Great staining

The Bad

None

The Bottom Line

consistent staining for BCL6 in both T cells and B cells

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