Great Staining for FoxP3

April 25, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor 532, eBioscience

Catalog Number:

58-5773-82

Clone Number:

FJK-16s

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

4deg 30min 1:250

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Great staining for FoxP3+ cells in secondary lymphoid organs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great staining

The Bad

None

The Bottom Line

Very consistent staining of FoxP3

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