Very Good Staining for F4/80

June 05, 2021

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-mouse F4/80 Antibody

Catalog Number:

123141

Clone Number:

BM8

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

4deg 30min 1:500

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

Great staining. Can detect F4/80+ cells among CD11b+ cells in the lymph nodes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very consistent quality

The Bad

None

The Bottom Line

Great clone to consistently stain for F4/80+ cells across multiple tissues.

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