Great staining for CCR7 in mouse

April 24, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cyanine5 anti-mouse CD197 (CCR7) Antibody

Catalog Number:

120114

Clone Number:

4B12

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

37deg for 1hr in RPMI

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Consistent staining of CCR7

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very consistent quality

The Bad

Have to incubate at 37deg for optimal staining

The Bottom Line

Very good signal for CCR7

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