Very Good Staining for CD45

April 27, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Pacific Blue™ anti-mouse CD45 Antibody

Catalog Number:

103126

Clone Number:

30-F11

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymocytes

Primary Incubation

4deg 30min 1:500

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Very good staining for CD45+ cells in the thymus

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very consistent quality

The Bad

None

The Bottom Line

Consistent and bright staining of CD45+ immune cells across multiple tissues.

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