Relatively Good Staining for TCRbeta

April 24, 2021

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

APC-Cy™7 Hamster Anti-Mouse TCR β Chain

Catalog Number:

560656

Clone Number:

H57-597

We are interested in characterizing the immune populations in secondary lymphoid organs under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

4deg 30min 1:100

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Decent antibody to detect TCRbeta+ T cells in the spleen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Distinct population of TCRb+ T cells

The Bad

None

The Bottom Line

Consistent staining

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