Boster
anti-CG6353 Q9VD92 Antibody
# DZ33921-1
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My lab is characterizing a mutant we named disjointed. The disjointed gene is also known as CG6353 or Archease. We have used this antibody for IF, and it appears to work well. It gives us a staining pattern for the endogenous protein that matches the pattern we see with an HA-tagged fusion protein detected by anti-HA staining.
Immunofluorescence
Drosophila larvae
1:100 at 4 degrees overnight
4% horse serum
1:1000 anti-Rb-A488
N/A
IF
Antibody staining reveals subcellular localization of the protein. In salivary gland and trachea, localization was primarily nuclear.
Polyclonal sera works with little background
May have to optimize staining
The only antibody to Drosophila archease available and it works