BD Biosciences
BUV395 Mouse Anti-Mouse CD45.2
564616
104
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CD45.2 is routinely used a congenic marker in mouse immunology as it is found all on all hematopoietic cells. We use this marker to identify donor T cells in our experiments when we perform adoptive transfer experiments.
Flow Cytometry
Murine tumor samples
1:500 in FACS Buffer (2% FBS/PBS)
FC Block in FACS buffer
N/A
Cytek Aurora (4 Laser (BVR, UV)
We can easily identify our donor T cells based on CD45.2 and CD45.1 expression in our tumor samples.
Titrate depending on cells since it can be too bright
Nice robust antibody that is readily available in UV from BD.
None
Recommend this antibody to use on the UV laser to detect CD45.2 expression.