Thermo Fisher Scientific
Hoechst 33258
33258
Info: Supplier Page
Cells were extensively washed with PBS1x before adding Hoechst 33258. A dilution of 1 uL/ml is working.If adding more, it is better to wash a lot with PBS, to avoid the strong background caused by the staining
microscopy
hela cells
None
The Hoechst 33258 stains the nuclei very well
N/A
Easy to optimize
Strong background
Nothing to say, this is one of my favorite product