Really Good Anti-mouse CD44

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse CD44 BV510

Catalog Number:

103043

Clone Number:

IM7

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD44 in B cells (CD19+).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

spleen of WT mice

Primary Incubation

anti-CD44 BV510 (1/100-200), 4°, 20'

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We can stain CD44 in B cells. In the figure, CD4 expression of CD44 is also depicted for control, there are several levels of CD44 in CD4 cells, and that can be observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable and good signal

The Bad

N/A

The Bottom Line

Very good option to stain mouse CD44

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