Great Alexa Fluor 546 Goat Anti-Rabbit

April 14, 2021

BlueskyReddit
Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Alexa Fluor 546 Goat Anti-Rabbit IgG (H+L)

Catalog Number:

11010

Clone Number:

N/A

We used this antibody as secondary antibody for IF in cell lines and mice tissue sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human cell lines and tissue

Primary Incubation

1:500 4 degree over night

Blocking Agent

3% BSA

Secondary Incubation

1:500

Tertiary Incubation

None

Detection

confocal microscopy

Results Summary

The signal is strong and minimal background could be detected. It works very well for IF and ICC.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable, high quality

The Bad

None

The Bottom Line

I recommend this for IF. It works well

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