Great Alexa Fluor 488 Goat Anti-Rabbit

April 23, 2021

BlueskyReddit
Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L)

Catalog Number:

11008

Clone Number:

N/A

We have been using this antibody as secondary antibody for IF or ICC. The signal is strong.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human cell lines and tissue

Primary Incubation

1:500 4 degree over night

Blocking Agent

3% BSA

Secondary Incubation

1:300

Tertiary Incubation

None

Detection

confocal microscopy

Results Summary

The signal is strong and minimal background could be detected. It works very well for IF and ICC.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable, high quality

The Bad

The price is high

The Bottom Line

I recommend this antibody as secondary antibody for IF and ICC.

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