BosterBio
Mettl23
Custom
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Browse Similar Products: Anti-Human METTL23 Western Blot Antibodies
This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low.
Western Blot
Human cell lines
1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking
5% BSA in TBS
Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking
None
Licor Odyssey IR Scanner
The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands.
The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery)
It gives consistent specific results
The bands in most cases are weak
An important addition to our analysis repertoire. We will definitely buy it again