Anti-CD19 APC for Flow Cytometry

March 04, 2021

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

anti-CD19 APC

Catalog Number:

340722

Clone Number:

SJ25C1

Info: Supplier Page 

Browse Similar Products: CD19 Flow Cytometry Antibodies

This antibody was used to stain for B cells in human peripheral blood. It was chosen because it is a commonly used clone for CD19, APC is bright and works well with other fluorophores in the staining panel, and BD has a good reputation for making quality flow cytometry antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood

Primary Incubation

20 ul per test in 100uL staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 0.2% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD19 staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well. Reproducible results.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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