Cell Signaling Technology
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
4858
D57.2.2E
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One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein.
Western Blot
Mouse brain
1:1000 dilution in 5% Milk prepared in 1x TBST
5% Milk prepared in 1x TBST
1:4000 dilution in 5% Milk prepared in 1x TBST
NONE
ECL
We found time dependent increase in Phospho-S6 Ribosomal Protein (Ser235/236) expression by chemoattractant.
No background and highly specific
None
This antibody works great for western blot and it is very specific. Signal is great. Highly recommended.