Biolegend
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
108125
D7
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining Sca-1 in plasma cells.
Flow Cytometry
spleen of a Blimp-1eGFP mouse
anti-Sca-1 APC-Cy7 (1/200), 20', 4°C
Fc block (2.4G2 clone)
N/A
Flow cytometer
Plasma cells (red) stained positive for Sca-1.
Reliable and good signal
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