Thermo Fisher Scientific
SOX2 Monoclonal Antibody (Btjce), eBioscience™
14-9811-80
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Browse Similar Products: SOX2 Immunohistochemistry Antibodies
Our lab uses this antibody to test for Sox2 expression in cochlear cells (Sox2 is the pro sensory domain marker in the developing cochlea then gets restricted to supporting cells in the auditory region of the inner ear). It works well on whole tissue and sections. An antigen retrieval step prior to immunostaining (15min @ 80 deg C) worked wonders in terms of staining clarity!
Immunohistochemistry
Mouse cochlea
1:250, primary incubation at 4 deg C overnight
10% normal goat serum
1:500, 2 hours at room temperature on shaker
None
Fluorescence microscope/confocal LSM 780 488 and/or 594nm
This is a one week old wildtype mouse cochlea section. The SOX2 staining here marks all supporting cells and few greater epithelial ridge cells very faintly as expected.
Not published yet
Works on a niche tissue like ear
None as of now
This is a good antibody for a small and niche tissue like the ear