Biolegend
Alexa Fluor 647 anti-mouse CD98
128210
RL388
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD98 in several B cell subsets.
Flow Cytometry
Blimp-1 eGFP mouse spleen
anti-CD98 A647 (1/400), 4°C, 20'
Fc block (2.4G2 clone)
N/A
Flow cytometry
We could nicely detect CD98 is plasma cells (Blimp-1+, red histogram), but not in B cells (blue histogram).
Reliable and good signal
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