ThermoFisher
Streptavidin, Alexa Fluor 647 conjugate
S21374
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+).
Flow Cytometry
Mouse WT Spleen
CD19 biot., 20min, 4°C
Fc block (2.4G2 clone)
SA-APC 1/3200, 4°C, 15 min
N/A
Flow cytometer
We could stain B cells (CD19+). This SA works really well, it can be used at 1/3200. Thus, it is really unexpensive.
Very good signal
I highly recomend to have this SA-APC, is it good and inexpensive.