Miltenyi
CD3 Antibody, anti-mouse, PE, REAfinity™
130-121-133
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The goal of this project was the investigation of the immune cell infiltrate in the murine skin during application of different stimulli by the MELC technology (Multi antigen staining). This antibody was used to visualize the CD3+ cells.
Immunofluorescence
Murine skin
1:20; RT; 60 min
5 % FBS; RT, 30 min
none
Fluorescence signals were detected by an inverted fluorescence microscope (Leica DM IRE2)
Antibody staining resulted in a great immunofluorescence image
Also a great antibody for IF (not only FC)
Recommendable!