Very Good CD8a Antibody for Immunofluorescence

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD8a Antibody Alexa Fluor 532

Catalog Number:

58-0081-80

Clone Number:

N/A

Aim of this project was the investigation of the immune cell infiltrate in the murine skin during application of different stimulli by the MELC technology. Here we stained for CD8a

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

Strong specific signals after few seconds of exposition. Any background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains CD8a very well

The Bad

N/A

The Bottom Line

Excellent staining

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