Reliable Antibody to Identify F8/40+ Cells

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

F4 /80 Antibody (CI-A3-1) [Alexa Fluor® 488]

Catalog Number:

NB600-404AF488

Clone Number:

N/A

Our lab was interested in the charaterization of leukocytes in differently treated murine skin sections. This antibody was used to visualize the the F4/80+ cells by direct immunfluorescence. The specimen was fresh frozen and fixed with acetone.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

Reliable results, easy to use

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great signal

The Bad

None

The Bottom Line

Good antibody. Results are reproducible.

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